| qRT-PCR |
pollens |
up-regulated |
N/A |
Zm401 is involved in the regulation of pollen development. Over expression of Zm401 in maize caused smaller tassels, degenerate or shriveled anthers. Zm401 may be involved in the development of male reproductive organs. Zm401, as a non-coding RNA, plays an essential role in pollen development. Zm401 gene is involved in the late stage of pollen development of maize. Zm401-overexpressed transgenic maize was generated to investigate its function in pollen development. (Dai et al., 2007) Zm401,is expressed specifically in pollens. Forward and reverse genetic studies have shown a function for Zm401 in regulating the expression of critical genes necessary for pollen development including ZmMADS2, MZm3-3 and ZmC5. MZm3-3 was up-regulated in Zm401 mutants while ZmMADS2 and ZmC5 were both down-regulated. Over-expression of Zm401 severely affects pollen development due to abnormal tassels and degenerate anthers. (Au et al., 2011) Zm401 is expressed primarily in the anthers (tapetal cells as well as microspores) in a developmentally regulated manner. That is, it is expressed from floret forming stage, increasing in concentration up to mature pollen. Knockdown of zm401 significantly affected the expression of ZmMADS2, MZm3-3, and ZmC5, critical genes for pollen development. zm401 could be one of the key growth regulators in anther development, the expression of zm401 is associated with the floret development, especially with the development of tapetal cells and microspores. (Ma et al., 2008) ZM401 was specifically or preferentially expressed in mature pollen. ZM401 was transcribed from tetrad stage of microspore development and increased in concentration up to mature pollen, which suggested that ZM401 belongs to lategene in pollen development. (Dai et al., 2004) |
21525783, 18465785, 400866 |
PLNlncRbase
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