LncRNA Information
ID EL0578 Name HOTAIR Aliases HOXAS; HOXC-AS4; HOXC11-AS1; NCRNA00072
Species Homo sapiens Chromosome 12 Start site 53962308
End site 53974956 Chain minus Exon NO. 7
Assembly Ensembl Release 89 Class antisense NCBI accession NR_047517, NR_047518, NR_003716
Ensembl ENSG00000228630 Sequence


Disease
Disease Method Sample Expression pattern Dysfunction type Description PMID Source
nasopharyngeal carcinoma knockdown nasopharyngeal carcinoma cells up-regulated interaction Hotair knockdown significantly attenuated both in vitro and in vivo tumor cell growth and angiogenesis. 26717040
esophageal squamous cell carcinoma lncRNA microarray, qRT-PCT esophageal squamous cell carcinoma (ESCC) tissue up-regulated expression ESCCAL_1 and HOTAIR may participate in the pathological process of ESCC. Furthermore, lncRNA could be potential diagnostic and prognostic biomarkers for ESCC. 25297587
prostate cancer microarray, qPCR etc. prostate cancer tissue, cell lines (LNCaP, PC3, DU145, RWPE-1 etc.) up-regulated N/A LncRNA profiling showed that HOTAIR was highly regulated by genistein and its expression was higher in castration-resistant PCa cell lines than in normal prostate cells. Knockdown (siRNA) of HOTAIR decreased PCa cell proliferation, migration and invasion and induced apoptosis and cell cycle arrest. miR-34a was also up-regulated by genistein and may directly target HOTAIR in both PC3 and DU145 PCa cells. Our results indicated that genistein inhibited PCa cell growth through down-regulation of oncogenic HOTAIR that is also targeted by tumor suppressor miR-34a. These findings enhance understanding of how genistein regulates lncRNA HOTAIR and miR-34a in PCa. 23936419 Lnc2Cancer
esophageal squamous cell carcinoma microarray, qPCR etc. ESCC tissue up-regulated expression Expression of HOTAIR lncRNA is increased in various cancers, and it is also significantly increased in our analysis of ESCC. 24222893 LncRNADisease Lnc2Cancer
non-functioning pituitary adenoma microarray, qPCR etc. NFPA tissue up-regulated expression Expression of the long non-coding RNAs MEG3, HOTAIR, and MALAT-1 in non-functioning pituitary adenomas and their relationship to tumor behavior. 24469926 LncRNADisease Lnc2Cancer
colorectal cancer microarray, qPCR etc. CRC tissue up-regulated regulation Two of the lncRNAs, HOTAIR and a novel lncRNA, lncRNA-422 were confirmed in more samples. GSEA indicated that gene sets most correlated with them were those named up-regulated in KRAS-over, down-regulated in JAK2-knockout, down-regulated in PDGF-over and down-regulated in TBK1-knockout, all of which were cancer-related. Subsequently, GO analyses of most significantly correlated coding genes of HOTAIR and lncRNA-422 showed that these two lncRNAs may participate in carcinogenesis by regulating protein coding genes involved in special biological process relevant to cancer. 25456707 Lnc2Cancer
triple-negative breast cancer microarray, qPCR etc. triple-negative breast cancer tissue up-regulated expression We found that the expression levels of TCONS_l2_00003938, ENST00000460164, ENST00000425295, MALAT1 and HOTAIR were significantly higher in tumor tissues than non-tumor tissues, whereas there were no significant differences in the expression levels of the other 3 lncRNAs. Our study identified a set of lncRNAs that were consistently aberrantly expressed in TNBC, and these dysregulated lncRNAs may be involved in the development and/or progression of TNBC. 25996380 Lnc2Cancer
papillary thyroid carcinoma microarray, qPCR etc. papillary thyroid carcinoma tissue up-regulated expression Expression profiles of five lnc-RNAs (MEG3, HULC, HOTAIR, NEAT1, and MALAT-1) previously shown to be involved in cancer metastasis were detected by qPCR in 5 pairs of papillary thyroid cancer and 11 matched lymph node metastatic tissues. Among the five, MEG3 showed significant down-expression. Overexpression of MEG3 inhibits thyroid cancer cell migration and invasion. 25997963 Lnc2Cancer
gastric cancer microarray, qPCR etc. gastric cancer tissue, cell lines (HCG-27, SGC-7901) up-regulated expression H19 and HOTAIR displayed significantly higher levels and the up-regulation of HOTAIR was associated with lymphatic metastasis and poor differentiation. 26237576 Lnc2Cancer
breast cancer microarray, qPCR, ISH etc. breast tumor tissue up-regulated N/A Co-expression of HOTAIR and EZH2 trended with a worse outcome. In matched primary and metastatic cancers, both HOTAIR and EZH2 had increased expression in the metastatic carcinomas. This approach offers a method to make observations on lncRNAs that may influence the cancer epigenome in a tissue-based technique. 23133536 Lnc2Cancer
hepatocelluar carcinoma microarray, qPCR, knockdown, Western blot etc. cell lines (HepG2 ,Bel-7402) up-regulated regulation Long non-coding RNA HOTAIR promotes cell migration and invasion via down-regulation of RNA binding motif protein 38 in hepatocellular carcinoma cells. 24663081 LncRNADisease Lnc2Cancer
cervical cancer microarray, qPCR, RIP etc. cervical cancer tissue, cell line (C33-A) down-regulated interaction We identified significant linear trend of progressive HOTAIR down-regulation through HPV negative controls, HPV16 positive non-malignants and CaCx samples. Majority of CaCx cases portrayed HOTAIR down-regulation in comparison to HPV negative controls, with corresponding up-regulation of HOTAIR target, HOXD10, and enrichment of cancer related pathways. However, a small subset had significantly higher HOTAIR expression, concomitant with high E7 expression and enrichment of metastatic pathways. Expression of HOTAIR and PRC2-complex members (EZH2 and SUZ12), showed significant positive correlation with E7 expression in CaCx cases and E7 transfected C33A cell line, suggestive of interplay between E7 and HOTAIR. 26152361 Lnc2Cancer
esophageal squamous cell carcinoma microarray, qPCR, Western blot, knockdown etc. ESCC tissue, cell lines (KYSE30, KYSE140, KYSE180, KYSE410, KYSE510) up-regulated N/A It was found that there was a great upregulation of HOTAIR in ESCC compared to their adjacent normal esophageal tissues. Meanwhile, patients with high HOTAIR expression have a significantly poorer prognosis than those with low expression. Moreover, HOTAIR was further validated to promote migration and invasion of ESCC cells in vitro. 24118380 Lnc2Cancer
glioma microarray, Western blot etc. glioblastoma tissue up-regulated N/A HOTAIR expression was significantly higher in HGG than in low-grade glioma (LGG; P < .001). Moreover, as shown in Fig. 1A, GBM demonstrated a significant increase in HOTAIR transcript levels, compared with that observed in normal tissues (P = .093), LGGs (P < .001), or AGs (P = .011). Our data establish that HOTAIR serves as a prognostic factor for glioma patient survival, as well as a biomarker for identifying glioma molecular subtypes, a critical regulator of cell cycle progression. 24203894 Lnc2Cancer
ovarian cancer monitored double-strand breaks recurrent platinum-resistant ovarian tumors vs primary ovarian tumors N/A N/A HOTAIR expression results in sustained activation of DNA damage response (DDR) after platinum treatment; HOTAIR regulates activation of NF-κB 27041570
gastric cancer N/A gastric cancer cell lines and tissue samples up-regulated locus/expression We found that the HOTAIR rs920778 TT carriers had a 1.66- and 1.87-fold increased gastric cancer risk in Jinan and Huaian populations compared with the CC carriers. 25640751
triple-negative breast cancer N/A Triple-negative breast cancer (TNBC) samples up-regulated expression HOTAIR has been known to induce tumor growth and metastasis in breast cancer. Depleting HOTAIR alone phenocopies the dual treatment in growth suppression. We show that expression of HOTAIR is regulated by β-catenin through a LEF1/TCF4-binding site. 25883211
LPS-induced sepsis N/A cardiomyocytes from sepsis mice up-regulated interaction HOTAIR silence preserved cardiac function of sepsis mice during LPS-induced sepsis. 26806307
Temporomandibular joint osteoarthritis N/A the synovial fluid of TMJ OA patients up-regulated N/A knockdown of HOTAIR in IL-1β-induced TMJ OA in vitro 27063559
breast cancer N/A N/A N/A expression HOTAIR showed positive association with 'hang Serum Response'. 19182780 LncRNADisease
breast cancer N/A N/A N/A expression A subsequent study revealed that HOTAIR is overexpressed in approximately one quarter of human breast cancers. 20930520 LncRNADisease
cancer N/A N/A N/A epigenetics Epigenetically silences gene expression at many loci by recruitment of LSD1/CoREST/REST and PRC2 repressive chromatin modifying complexes. Oncogene: promotes tumour metastasis. 21256239 LncRNADisease
breast cancer N/A N/A N/A expression The lncRNA HOTAIR is a strong predictor of metastasis in breast tumors and its over-expression in various breast carcinoma cell lines promotes invasion. 21903344 LncRNADisease
breast cancer N/A N/A N/A expression Elevated expression of HOTAIR is observed in primary and metastatic breast cancer. 21925379 LncRNADisease
cancer N/A N/A N/A expression HOTAIR is causally involved in tumor progression. It is significantly overexpressed in metastatic breast cancer. 22045689 LncRNADisease
breast cancer N/A N/A N/A regulation On a more mechanistic level, recent studies have revealed the contribution of lncRNAs as proto-oncogenes, e.g. GAGE6, as tumor suppressor genes, e.g. 鈥榩15 antisense RNA and lincP21' (36,91), as drivers of metastatic transformation, e.g. HOTAIR in breast cancer, and as regulators of alternative splicing, e.g. MALAT1 22492512 LncRNADisease
breast cancer N/A N/A N/A expression The wellstudied lincRNA, HOTAIR, is highly expressed in breast cancer metastases, and its overexpression in various breast carcinoma cell lines promotes invasion 22535282 LncRNADisease
breast cancer N/A N/A N/A expression Overexpression of the HOTAIR transcript, a cis-lncRNA associated with the HOXD gene cluster, has been associated with hepatocellular carcinoma [32], colorectal cancer [33] and breast cancer [13] by deregulation of HOXD cluster genes 22817756 LncRNADisease
colorectal cancer N/A N/A N/A expression Overexpression of the HOTAIR transcript, a cis-lncRNA associated with the HOXD gene cluster, has been associated with hepatocellular carcinoma [32], colorectal cancer [33] and breast cancer [13] by deregulation of HOXD cluster genes 22817756 LncRNADisease
hepatocelluar carcinoma N/A N/A N/A expression Overexpression of the HOTAIR transcript, a cis-lncRNA associated with the HOXD gene cluster, has been associated with hepatocellular carcinoma [32], colorectal cancer [33] and breast cancer [13] by deregulation of HOXD cluster genes 22817756 LncRNADisease
breast cancer N/A N/A N/A regulation Gene silencing by interacting with PRC2 and LSD1/CoREST complexes. 22996375 LncRNADisease
cancer N/A N/A N/A expression HOTAIR overexpression is associated with poor prognosis in breast (Gupta et al. 2010), liver (Z. Yang et al. 2011), colorectal (Kogo et al. 2011), gastrointestinal (Niinuma et al. 2012), and pancreatic (Kim et al. 2012) cancers and is proposed to increase tumor invasiveness and metastasis (Gupta et al. 2010). 23463798 LncRNADisease
cancer N/A N/A N/A expression HOTAIR overexpression has been linked to increased invasiveness and poorer outcomes in several human cancers. 23473599 LncRNADisease
breast cancer N/A N/A N/A expression Recent studies have linked their mis-expression to diverse cancers (ANRIL: prostate cancer, XIST: female cancers, HOTAIR: breast cancer, KCNQ1OT3: colorectal cancer). 23660942 LncRNADisease
hepatocelluar carcinoma N/A N/A N/A expression Dysregulation and functional roles of lncRNAs in HCC 24183851 LncRNADisease
breast cancer N/A N/A N/A regulation Epigenetically silences gene expression via LSD1/CoREST & PRC2; metastasis 24499465 LncRNADisease
breast cancer N/A N/A N/A expression HOTAIR was proposed as a diagnostic marker in breast and colorectal cancer. Its depletion resulted in reduced invasiveness, and its expression level correlated with differentially regulated genes of the PRC2 complex. 24531795 LncRNADisease
colorectal cancer N/A N/A N/A expression HOTAIR was proposed as a diagnostic marker in breast and colorectal cancer. Its depletion resulted in reduced invasiveness, and its expression level correlated with differentially regulated genes of the PRC2 complex. 24531795 LncRNADisease
breast cancer N/A N/A N/A expression In approximately one-quarter of human breast cancers, HOTAIR is highly induced, while its elevated levels are also predictive of metastasis and disease progression in other cancers, such as colon, colorectal, gastrointestinal, pancreatic and liver cancer. 24667321 LncRNADisease
colon cancer N/A N/A N/A expression In approximately one-quarter of human breast cancers, HOTAIR is highly induced, while its elevated levels are also predictive of metastasis and disease progression in other cancers, such as colon, colorectal, gastrointestinal, pancreatic and liver cancer. 24667321 LncRNADisease
colorectal cancer N/A N/A N/A expression In approximately one-quarter of human breast cancers, HOTAIR is highly induced, while its elevated levels are also predictive of metastasis and disease progression in other cancers, such as colon, colorectal, gastrointestinal, pancreatic and liver cancer. 24667321 LncRNADisease
gastrointestinal cancer N/A N/A N/A expression In approximately one-quarter of human breast cancers, HOTAIR is highly induced, while its elevated levels are also predictive of metastasis and disease progression in other cancers, such as colon, colorectal, gastrointestinal, pancreatic and liver cancer. 24667321 LncRNADisease
liver cancer N/A N/A N/A expression In approximately one-quarter of human breast cancers, HOTAIR is highly induced, while its elevated levels are also predictive of metastasis and disease progression in other cancers, such as colon, colorectal, gastrointestinal, pancreatic and liver cancer. 24667321 LncRNADisease
pancreatic cancer N/A N/A N/A expression In approximately one-quarter of human breast cancers, HOTAIR is highly induced, while its elevated levels are also predictive of metastasis and disease progression in other cancers, such as colon, colorectal, gastrointestinal, pancreatic and liver cancer. 24667321 LncRNADisease
breast cancer N/A N/A N/A expression For example, HOTAIR is remarkably overexpressed in breast tumors and the expression of HOTAIR in primary breast tumors is a strong prognosis marker of patient outcomes such as metastasis and patient survival 24721780 LncRNADisease
rheumatoid arthritis N/A N/A N/A regulation PBMC and exosome-derived Hotair is a critical regulator and potent marker for rheumatoid arthritis. 24722995 LncRNADisease
cancer N/A N/A N/A expression Overexpression of HOTAIR was found in breast and colon cancers and was associated with metastasis and poor prognosis. 24757675 LncRNADisease
esophageal squamous cell carcinoma N/A N/A N/A expression Upregulation of HOTAIR is associated with metastasis of gastric cancer, lung cancer, and esophageal squamous cell carcinoma? 24757675 LncRNADisease
gastric cancer N/A N/A N/A expression Upregulation of HOTAIR is associated with metastasis of gastric cancer, lung cancer, and esophageal squamous cell carcinoma? 24757675 LncRNADisease
lung cancer N/A N/A N/A expression Upregulation of HOTAIR is associated with metastasis of gastric cancer, lung cancer, and esophageal squamous cell carcinoma? 24757675 LncRNADisease
esophageal squamous cell carcinoma N/A N/A N/A expression The result showed that the expression levels of HOTAIR were upregulated in samples from patients with higher tumor burdens, which were defined as those with larger tumors, advanced clinical staging, increased lymph node tumor burdens and the presence of distant metastases.? 24817925 LncRNADisease
laryngeal squamous cell carcinoma N/A N/A N/A expression Inspired observed that HOTAIR was higher expressed in primary LSCC than in adjacent noncancerous tissues. It is noteworthy that over-expression of HOTAIR was related to poor differentiation, lymph node metastasis, or advanced clinical stages of LSCC. The results suggested that HOTAIR promotes the malignant progression of LSCC. 24817925 LncRNADisease
nasopharyngeal carcinoma N/A N/A N/A expression The result showed that, the expression levels of HOTAIR wereup-regulated in NPC tissues than in non-cancerous tissues. Further study demonstrated that HOTAIR was up-regulated in NPC cell lines with high invasive potential and the capacity for migration, invasion and proliferation was suppressed after knocking down HOTAIR expression. 24817925 LncRNADisease
oral squamous cell carcinoma N/A N/A N/A expression Subsequently, they confirmed that the expression levels of HOTAIR, NEAT-1 and UCA1 in metastasized samples was prominent higher than the non-metastatic samples.? 24817925 LncRNADisease
breast cancer N/A N/A N/A regulation Gupta et al found that lncRNA HOTAIR overexpression was a strong predictor of breast tumor metastasis. 24829860 LncRNADisease
cancer N/A N/A N/A regulation Forced expression of HOTAIR in epithelial cancer cells altered the localization of PRC2 on chromatin. Genome-wide studies revealed PRC2 localization more resembling occupancy in embryonic fibroblasts.? 24829860 LncRNADisease
lung adenocarcinoma N/A N/A N/A regulation In this study, the lncRNA HOTAIR was upregualted in lung adenocarcinoma cells grown in 3-D cell culture supplemented with collagen.? 24829860 LncRNADisease
gastric cardia adenocarcinoma PCR-RFLP, qPCR etc. blood up-regulated mutation Among three htSNPs of the HOTAIR gene (rs12826786 C>T, rs4759314 A>G, and rs10783618 C>T), only the T allele of rs12826786 was found to increase the risk of developing GCA and was associated with smoking habit and tumor-node-metastasis (TNM) stage. In addition, higher expression levels of HOTAIR were found in tumor tissues and rs12826786 SNP has a genotype-specific effetc on HOTAIR expression. A high HOTAIR expression level was associated with poor GCA patients' survival. 25476857 Lnc2Cancer
breast cancer polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) and created-restriction-site PCR (CRS-RFLP) assays, multifactor dimensionality reduction (MDR) method N/A N/A mutation Subjects with Trs920778 had a significantly increased risk of breast cancer (OR: 1.41, 95%CI: 1.13, 1.75). We observed a significant interaction between rs920778 and reproductive factors, including age at menopause, number of abortions, and family history. Genetic variant rs920778 in HOTAIR significantly increased the risk of BC, and it may have apparent interaction with reproductive factors in the progression on BC. 26547792
gastrointestinal stromal tumor qPCR etc. gastric cancer tissue up-regulated expression Overexpression of HOTAIR was also strongly associated with high-risk grade and metastasis among GIST specimens. 22258453 LncRNADisease Lnc2Cancer
cervical cancer qPCR etc. cell line (CaSki) down-regulated N/A Generally, we found that some of the RNA molecules (HOTAIR and MALAT1) are down-regulated while many of them (lincRNA-p21, GAS5, MEG3, ANRIL, and ncRNA-CCND1) are up-regulated and some others (TUG1, UCA1, and PANDA) not affected. The decline in the expression of HOTAIR and MALAT1 was clearly evident in BLM-treated HeLa and MCF cells. For lincRNA-p21, ncRNA-CCND1, and MEG3, a similar up-regulation pattern was obvious in both cell lines where the increase was generally more pronounced in BLM-treated cells. 22487937 LncRNADisease Lnc2Cancer
breast cancer qPCR etc. breast cancer tissue differential expression N/A A positive correlation was found between DNA methylation and HOTAIR expression. Methylation was associated with unfavorable disease characteristics, whereas no significant associations were found between HOTAIR expression and clinical or pathologic features. In multivariate, but not in univariate, Cox proportional hazard regression models, patients with high HOTAIR expression had lower risks of relapse and mortality than those with low HOTAIR expression. We found that patients with high levels of HOTAIR expression had lower risks of relapse and death than those with low expression. The results suggest that clinicopathological features and therapy treatments could modify the effect of HOTAIR. Large noncoding RNA HOTAIR, transcribed from the antisense strand of HOXC12, interacts with Polycomb Repressive Complex 2 (PRC2) in the regulation of gene activities. 23124417 Lnc2Cancer
oral squamous cell carcinoma qPCR etc. OSCC tissues up-regulated N/A We found that most of the selected transcripts (4/6) were upregulated in tumors relative to matched adjacent nonmalignant tissue. One gene, MEG-3, was downregulated in cancer compared with its adjacent nonmalignant tissue. Expression of lncRNA (HOTAIR, NEAT-1 and UCA1) was significantly higher in the samples that subsequently metastasized compared with the non-metastatic samples. By contrast, MEG-3 was downregulated in the metastatic samples. These findings suggest that the detection of lncRNAs in saliva may be used as a noninvasive and rapid diagnostic tool for the diagnosis of oral cancer. 23292713 Lnc2Cancer
hepatocelluar carcinoma qPCR etc. HCC tissue, cell lines (HepG2 cell line) up-regulated expression Clinical significance of the expression of long non-coding RNA HOTAIR in primary hepatocellular carcinoma. 23292722 LncRNADisease Lnc2Cancer
ovarian cancer qPCR etc. ovarian cancer tissue up-regulated N/A The expression of HOTAIR in ovarian cancer tissue was higher than that in normal ovarian tissue. The expression was statistically higher in poorly differentiated ovarian cancer than poorly-moderately, moderately-well, and well-differentiated ones (1.65 +/- 0.41 vs. 0.39 +/- 0.14, P < 0.05). 23600210 Lnc2Cancer
lung cancer qPCR etc. lung cancer tissue cell lines (A549, mK-ras-LE etc.) up-regulated N/A In the current study, we demonstrate that the tumor-promoting lincRNA HOTAIR is induced by Col-1 and its expression inversely correlates with acinar morphogenesis, a differentiation feature of lung epithelial cells in rBM 3-D culture. These in vitro findings suggest that the elevated HOTAIR expression in tumor tissues results from cancer cells' response to Col-1 that is aberrantly enriched in the tumor microenvironment. Our findings indicate that tumor-promoting Col-1 up-regulates the expression of HOTAIR in NSCLC cells. These initial results warrant further investigation of HOTAIR and other lincRNA genes in lung tumorigenesis. 23668363 Lnc2Cancer
non-small cell lung cancer qPCR etc. NSCLC tissue, cell lines (A549) up-regulated N/A High expression of HOTAIR (tumor/normal ratio >= 2) was detected in 17 patients (22.1%) and was frequently found in patients with advanced stage, lymph node metastasis or lymph-vascular invasion and short disease free interval. Furthermore, brain metastases show significantly higher HOTAIR expression compared to primary cancer tissues. HOTAIR-expressing A549 cells showed induced cell migration and anchorage independent cell growth in vitro. 23743197 Lnc2Cancer
gastric adenocarcinoma qPCR etc. gastric adenocarcinoma tissue up-regulated N/A Here, we found aberrant up-regulation of HOTAIR in gastric adenocarcinoma samples compared with normal adjacent gastric epithelium tissues. Besides, we found that the aberrant expression of HOTAIR was associated with TNM staging and lymph node metastasis of gastric tumors. Here, a potential cooperative expression between HOTAIR SUZ12 genes in gastric adenocarcinoma tissues is deduced. This result suggests a role for HOTAIR long noncoding RNA in gastric cancer progression. 23888369 Lnc2Cancer
endometrial cancer qPCR etc. endometrial carcinoma tissue up-regulated expression The long non-coding RNA HOTAIR is upregulated in endometrial carcinoma and correlates with poor prognosis. 24285342 LncRNADisease Lnc2Cancer
pituitary adenoma qPCR etc. pituitary adenomas tissue up-regulated expression Expression of the long non-coding RNAs MEG3, HOTAIR, and MALAT-1 in non-functioning pituitary adenomas and their relationship to tumor behavior. 24469926 LncRNADisease Lnc2Cancer
multiple myeloma qPCR etc. blood (plasma) down-regulated expression HOTAIR long non-coding RNA is a negative prognostic factor not only in primary tumors, but also in the blood of colorectal cancer patients. 24583225 LncRNADisease Lnc2Cancer
colorectal cancer qPCR etc. colorectal cancer tissue, blood up-regulated regulation HOTAIR long non-coding RNA is a negative prognostic factor not only in primary tumors, but also in the blood of colorectal cancer patients. 24583926 LncRNADisease Lnc2Cancer
cervical cancer qPCR etc. cervical cancer tissue up-regulated expression Overexpression of?long?noncoding?RNA?HOTAIR predicts a poor prognosis in patients with cervical cancer. 24748337 LncRNADisease Lnc2Cancer
gastric cancer qPCR etc. gastric cancer tissue up-regulated N/A The expression of HOTAIR was markedly increased in gastric cancer tissues compared with adjacent non-tumoral tissues. We further showed that there was a positive significant correlation between the HOTAIR gene expression, TNM staging, perineural invasion, and distant metastasis, but not with other clinicopathological features of gastric tumors. HOTAIRexpression is modulated during gastric cancer progression and therefore may participate in molecular processes relevant to malignant transformation and metastasis in gastric carcinoma. 24949306 Lnc2Cancer
laryngeal squamous cell carcinoma qPCR etc. LSCC tissue up-regulated expression We discovered that five lncRNAs were differentially expressed between primary LSCC samples and adjacent normal tissues. Among them, three lncRNAs were up-expressed in tumor specimens, including CDKN2B-AS1, HOTAIR and MALAT1. More, two lncRNAs had significant down-expression, which were lncRNA RRP1B and SRA1. Cisplatin and paclitaxel have target function on significant lncRNAs in LSCC, which presents novel molecular targets to cure LSCC patients and also leads an orientation for developing new drugs. 25257554 Lnc2Cancer
breast cancer qPCR etc. cells lines (MCF-7) up-regulated interaction When compared with MCF-7 cells, MCF-7-TNR cells exhibited an increase in the expression of HOTAIR, which correlated with characteristics of a luminal-like to basal-like transition as evidenced by dysregulated gene expression and accelerated growth. MCF-7-TNR cells exhibited reduced suppressive histone H3 lysine27 trimethylation on the HOTAIR promoter. Inhibition of HOTAIR and EZH2 attenuated the luminal-like to basal-like transition in terms of gene expression and growth in MCF-7-TNR cells. HOTAIR was robustly expressed in the native basal-like breast cancer cells and inhibition of HOTAIR reduced the basal-like gene expression and growth. 25328122 Lnc2Cancer
cervical cancer qPCR etc. blood (serum) up-regulated expression Compared with normal control, the expression of HOTAIR was significantly upregulated in the sera of cervical cancer patients. In addition, elevated HOTAIR was associated with advanced tumor stages, adenocarcinoma, lymphatic vascular space invasion, and lymphatic node metastasis. In addition, our follow-up data showed that high HOTAIR was notably correlated with tumor recurrence and short overall survival. 25366139 Lnc2Cancer
gastric cancer qPCR etc. gastric cancer tissue, cell lines(AGS, MKN45, 7901 etc.) up-regulated expression All the 8 lncRNAs were then subjected to qPCR validation using 20 pairs of GC and control tissues. Among them, HOTAIR, PVT1, H19, MALAT1, GHET1 and HULC were significantly higher in tumor tissues compared with control tissues. 26096073 Lnc2Cancer
gastric adenocarcinoma qPCR etc. gastric cancer tissue up-regulated expression HOTAIR was significantly upregulated in GA tissues, especially in more advanced cases. High HOTAIR expression was an independent poor prognostic factor for patients with advanced GA. Further stratification analyses revealed that the association between HOTAIR expression and survival in patients with advanced GA remained significant in the subgroup of patients with TNM stages IIIA and IIIB, poorly differentiated, and smaller tumors. 26328013 Lnc2Cancer
ovarian cancer qPCR etc. ovarian cancer tissue up-regulated expression HOTAIR expression was significantly associated with poor survival in carboplatin-treated patients with adjusted hazard ratios for death of 3.64 in the discovery and 1.63 in the validation set. This effect was not seen in patients who did not receive carboplatin. HOTAIR expression or its surrogate DNAme signature predicted poor outcome in all additional sets of carboplatin-treated ovarian cancer patients while HOTAIR expressors responded preferentially to cisplatin. 26497652 Lnc2Cancer
breast cancer qPCR, ChIP-chip etc. cell lines (SUZ12, EZH2, PRC2 etc.) up-regulated expression HOTAIR expression level in primary tumors is a powerful predictor of eventual metastasis and death. 20393566 LncRNADisease Lnc2Cancer
laryngeal squamous cell carcinoma qPCR, in vitro knockdown etc. cell lines (Hep-2) up-regulated N/A HOTAIR levels were significantly higher in LSCC than in corresponding adjacent non-neoplastic tissues, and patients with poor histological grade or advanced clinical stage had higher HOTAIR expression. PTEN methylation was significantly reduced in Hep-2 cells depleted of HOTAIR. Taken together, these results suggest that the oncogenic role of HOTAIR in LSCC is related to promotion of PTEN methylation. 23141928 Lnc2Cancer
melanoma qPCR, in vitro knockdown etc. melanoma tissue, cell lines (A375 etc.) up-regulated N/A Among the tested lncRNAs, HOTAIR was the most highly expressed in lymph node metastasis. The role of HOTAIR in melanoma cell motility and invasion was further evaluated by knocking down HOTAIR with siRNAs. Knockdown of HOTAIR resulted in the reduction of motility and invasion of human melanoma cell line A375, as assessed by wound healing assay and Matrigel-based invasion assay. siHOTAIR also suppressed the degradation of gelatin matrix, suggesting that HOTAIR promotes gelatinase activity. Together, our study shows thatHOTAIR is overexpressed in metastatic tissue, which is associated with the ability of HOTAIR to promote melanoma cell motility and invasion. 23862139 Lnc2Cancer
esophageal squamous cell carcinoma qPCR, in vitro knockdown etc. ESCC tissu, cell lines (TE1, TE3, TE7, TE8, KYSE30, KYSE180 etc.) up-regulated regulation Long non-coding RNA HOTAIR, a driver of malignancy, predicts negative prognosis and exhibits oncogenic activity in oesophageal squamous cell carcinoma. 24022190 LncRNADisease Lnc2Cancer
nasopharyngeal carcinoma qPCR, ISH etc. primary NPC tissue up-regulated N/A Quantified using qPCR, HOTAIR expression levels in fresh tissue and paraffin-embedded samples were 5.2 ~ 48.4-fold higher compared with non-cancer tissue samples. Moreover, HOTAIR expression levels increased with clinical stage progression, which was consistent with ISH findings in the paraffin-embedded tissue. Most importantly, NPC patients with higher HOTAIR levels had a poor prognosis for overall survival using univariate and multivariate analysis. In addition, HOTAIR mediated the migration, invasion and proliferation of NPC cells in vitro. 23281836 Lnc2Cancer
esophageal squamous cell carcinoma qPCR, ISH etc. ESCC tissue up-regulated N/A High expression levels of HOTAIR in ESCC patients correlated positively with clinical stage, TNM classification, histological differentiation and vital status. HOTAIR expression was found to be an independent prognostic factor in ESCC patients. ESCC patients who expressed high levels of HOTAIR had substantially lower overall 5-year survival rates than HOTAIR-negative patients. In vitro assays of ESCC cell lines demonstrated that HOTAIR mediated the proliferation, colony formation and migratory capacity of ESCC cells. 23717443 Lnc2Cancer
breast cancer qPCR, ISH, RIP, ChIP etc. breast cancer tissue, cell lines (MCF7, T47D) up-regulated interaction In this study, we report that HOTAIR (HOX antisense intergenic RNA) is upregulated in tamoxifen-resistant breast cancer tissues compared to their primary counterparts. Mechanistically, HOTAIR is a direct target of ER-mediated transcriptional repression and is thus restored upon the blockade of ER signaling, either by hormone deprivation or by tamoxifen treatment. Interestingly, this elevated HOTAIR increases ER protein level and thus enhances ER occupancy on the chromatin and potentiates its downstream gene regulation. 26364613 Lnc2Cancer
hepatocelluar carcinoma qPCR, knockdown etc. HCC tissue, cell lines (SMMC-7721, HepG2, Hep3B etc.) up-regulated expression Overexpression of long non-coding RNA HOTAIR predicts tumor recurrence in hepatocellular carcinoma patients following liver transplantation. 21327457 LncRNADisease Lnc2Cancer
colorectal cancer qPCR, knockdown etc. CRC tissue, (HEK293T, HCT116, SW480) up-regulated epigenetics Long noncoding RNA HOTAIR regulates polycomb-dependent chromatin modification and is associated with poor prognosis in colorectal cancers.patients with high HOTAIR expression had a relatively poorer prognosis. 21862635 LncRNADisease Lnc2Cancer
gastric cancer qPCR, knockdown etc. gastric cancer tissue, cell lines(MKN74, KATO III etc.) up-regulated N/A The expression of HOTAIR was significantly higher in cancer lesions than in adjacent non-cancerous tissues in human gastric cancers. In the diffuse type of gastric cancer, the High-HOTAIR group (HOTAIR/GAPDH > 1) showed significantly more venous invasion, frequent lymph node metastases and a lower overall survival rate compared to the Low-HOTAIR group (HOTAIR/GAPDH < 1). 24130837 Lnc2Cancer
esophageal squamous cell carcinoma qPCR, knockdown etc. ESCC tissue, cell lines (KYSE30, KYSE150, KYSE450, KYSE510, KYSE180 etc.) up-regulated N/A Notably elevated HOTAIR expression levels were observed in cancerous tissues compared to adjacent noncancerous tissues (96%, P < 0.01), showing a high correlation with cancer metastasis (P < 0.01), elevated TNM (2009) stage classification (P < 0.01), and lowered overall survival rates (P = 0.003). Multivariate analysis revealed that HOTAIR expression (P = 0.003) is also an independent prognostic factor for comparison of TNM stage (P = 0.024) and lymph node metastasis (P = 0.010). 24151120 Lnc2Cancer
small cell lung cancer qPCR, knockdown etc. cell lines (COLO-668, COR-L51, COR-L88, DMS-79, DMS-53 etc.) up-regulated regulation Long noncoding RNA HOTAIR is relevant to cellular proliferation, invasiveness, and clinical relapse in small-cell lung cancer. 24591352 LncRNADisease Lnc2Cancer
endometrial cancer qPCR, knockdown etc. endometrioid carcinoma tissues, cell lines (HEC-1A, HEC-1B, Ishikawa, RL-952) up-regulated regulation Lentivirus-Mediated?RNA?Interference Targeting the?Long?Noncoding?RNA?HOTAIR Inhibits Proliferation and Invasion of Endometrial Carcinoma Cells In Vitro and In Vivo. 24758900 LncRNADisease Lnc2Cancer
lung cancer qPCR, knockdown etc. lung cancer tissue up-regulated N/A Among the tested lncRNAs, HOTAIR and NEAT1 were most highly expressed in lymph node metastasis. However, only HOTAIR was subsequently found to be involved in lung cancer cell motility and invasion, as assessed by in vitro migration and invasion assay. 25010625 Lnc2Cancer
gastric cancer qPCR, knockdown etc. gastric cancer tissue, cell lines (MKN7, MKN45, MKN74, NUGC4, AZ521, AGS, KATOIII) up-regulated interaction Expression of both lncRNAs was significantly higher in cancerous tissues than in corresponding normal mucosa, and higher expression of these lncRNAs significantly correlated with peritoneal metastasis in GC patients. In addition, elevated HOTAIR expression emerged both as an independent prognostic and risk factor for peritoneal dissemination. SiRNA knockdown of HOTAIR in GC cells significantly inhibited cell proliferation, migration and invasion, but concurrently enhanced the anoikis rate in transfetced cells. 25280565 Lnc2Cancer
colorectal cancer qPCR, knockdown etc. CRC tissue up-regulated expression Our study showed that genetic variants in HOTAIR were associated with risk of colorectal cancer and rs7958904 may act as a potential biomarker for predicting the risk of colorectal cancer. 25432874 Lnc2Cancer
serous ovarian cancer qPCR, knockdown etc. cell lines (A2780, Hey, SKOV3, OVCA429, OVCA433, OVCAR3) up-regulated expression We found that HOTAIR levels were overexpressed in SOC tissues compared with normal controls and that HOTAIR overexpression was correlated with an advanced FIGO stage and a high histological grade. HOTAIR knockdown resulted in the induction of cell cycle arrest and apoptosis through certain cell cycle-related and apoptosis-related proteins. 25796453 Lnc2Cancer
acute myeloid leukemia qPCR, knockdown etc. bone marrow, cell lines (HL60, K562) up-regulated expression We found that HOTAIR is significantly upregulated in de novo AML patients compared with those of AML-CR patients and normal controls; the reduction of HOTAIR by small interfering RNA (siRNA) repressed the proliferation of HL-60 and K562; the higher expression level of HOTAIR in AML patients was significantly correlated with NCCN high risk group. 26261618 Lnc2Cancer
lung adenocarcinoma qPCR, knockdown etc. NSCLC and adjacent non-tumor lung tissues, cell lines (A549, PC9, H1299, H520, HBE) up-regulated interaction HOTAIR regulates the ratio of FOXA1 to FOXA2 and migration and invasion. HOTAIR and the ratio of FOXA1 to FOXA2 are negatively correlated. HOTAIR knockdown inhibits migration and invasion. HOTAIR is associated with LSH, and this association linked with the binding of LSH in the promoter of FOXA1, not FOXA2. Targeted inhibition of HOTAIR suppresses the migratory and invasive properties 26658322 Lnc2Cancer
pancreatic cancer qPCR, Luciferase reporter assay, in vitro knockdown etc. cell lines (Panc1, MiaPaCa2, Panc28, L3.6pl) up-regulated N/A We show that HOTAIR expression is increased in pancreatic tumors compared with non-tumor tissue and is associated with more aggressive tumors. Knockdown of HOTAIR (siHOTAIR) by RNA interference shows that HOTAIR has an important role in pancreatic cancer cell invasion, as reported in other cancer cell lines. HOTAIR knockdown in Panc1 and L3.6pL pancreatic cancer cells that overexpress this lincRNA decreased cell proliferation, altered cell cycle progression and induced apoptosis, demonstrating an expanded function of HOTAIR in pancreatic cancer cells compared with other cancer cell lines. HOTAIR uniquely suppressed several interferon-related genes and gene sets related to cell cycle progression in pancreatic cancer cells and tumors. 22614017 Lnc2Cancer
gastric cancer qPCR, Luciferase reporter assay, knockdown, ELISA etc. gastric cancer tissue, cell lines (SGC7901, MGC-803) up-regulated interaction Significant HOTAIR overexpression was observed in GC tissues, as well as strong positive correlations with HLA-G levels in both tissue and peripheral blood samples. HOTAIR overexpression might also get involved in tumor escape mechanisms, involving HLA-G upregulation via inhibiting miR-152. 26187665 Lnc2Cancer
gallbladder cancer qPCR, Northern blot, in vitro knockdown, RIP etc. gallbladder cancer tissue, cell lines (GBC-SD, SGC-996,NOZ, EH-GB2 etc.) up-regulated N/A The HOTAIR transcripts were expressed at higher levels in the tumor tissues compared with adjacent normal tissues (p < 0.0001), indicating that HOTAIR was frequently up-regulated in GBC.A positive correlation between c-Myc and HOTAIR mRNA levels was observed in gallbladder cancer tissues. HOTAIR is a c-Myc-activated driver of malignancy, which acts in part through repression of miRNA-130a. 24953832 Lnc2Cancer
gastric cancer qPCR, Northern blot, knockdown etc. gastric cancer tissue up-regulated N/A HOTAIR Long Noncoding RNA Promotes Gastric Cancer Metastasis through Suppression of Poly r(C)-Binding Protein (PCBP) 1. Our findings provide mechanistic evidence for HOTAIR overexpression and PCBP1 downregulation and the ensuing malignant phenotype in both cultured and xenograft gastric cancer cells. 25612617 LncRNADisease Lnc2Cancer
prostate cancer qPCR, RNA Pull-Down Assay, RIP, ChIP etc. prostate cancer tissue up-regulated interaction Here, we report HOTAIR as an androgen-repressed lncRNA, and, as such, it is markedly upregulated following androgen deprivation therapies and in CRPC. Functionally, HOTAIR overexpression increases, whereas HOTAIR knockdown decreases, prostate cancer cell growth and invasion. Taken together, our results provide compelling evidence of lncRNAs as drivers of androgen-independent AR activity and CRPC progression, and they support the potential of lncRNAs as therapeutic targets. 26411689 Lnc2Cancer
aortic valve calcification qPCR, siRNA, DNA microarray BAVs, human aortic interstitial cells (AVICs) down-regulated expression Reducing HOTAIR levels via siRNA in AVICs results in increased expression of calcification genes. 24788418
sarcoma qPCR, Western blot etc. Primary and metastatic sarcoma tumor tissue up-regulated N/A High level expression of both of MTDH/AEG1 and HOTAIR in the primary tumor correlated with a likelihood to metastasize. High levels of both MTDH/AEG-1 and HOTAIR in primary sarcoma are correlated with a high probability of metastasis. By contrast, reduced expression of both MTDH/ AEG-1 and HOTAIR is correlated with a good response to treatment in terms of necrosis, suggesting that levels of MTDH and HOTAIR are potential biomarkers for treatment efficacy. 23543869 Lnc2Cancer
bladder cancer qPCR, Western blot etc. bladder cancer tissue, cell lines (T24, J82, BIU-87 etc.) up-regulated expression Ninety out of 110 specimens were detected in HOTAIR high expression. Histological grade and expression levels of HOTAIR were positively correlated with the recurrence rate. HOTAIR expression (hazard ratio 4.712; 95 % CI 2.894-8.714; P < 0.001) was an independent predictor of recurrence rate in multivariate Cox regression analysis. HOTAIR expression is correlated with patients' poor prognosis. 25030736 Lnc2Cancer
gastric cancer qPCR, Western blot etc. gastric cancer tissue, gastric cell lines up-regulated interaction The expression of HOTAIR was significantly elevated in various gastric cancer cell lines and tissues compared to normal control. Lymphovascular invasion and lymph node metastasis were more common in the high level of HOTAIR group. si-HOTAIR significantly decreased invasiveness and migration. si-HOTAIR led to differential expression of epithelial to mesenchymal transition markers. HOTAIR was involved in inhibition of apoptosis and promoted invasiveness, supporting a role for HOTAIR in carcinogenesis and progression of gastric cancer. 25063030 Lnc2Cancer
laryngeal squamous cell carcinoma qPCR, Western blot etc. blood (serum) up-regulated N/A The expression of exosomal miR-21 and HOTAIR was significantly higher in patients with laryngeal squamous cell carcinomaLSCC than those with vocal cord polyps; The patients with lymph node metastasis had higher serum exosomal miR-21 and HOTAIR expressions than those without. 25099764 LncRNADisease Lnc2Cancer
cervical cancer qPCR, Western blot etc. cervical cancer tissue, cell lines (HeLa, SiHa, C33A, CaSki) differential expression regulation Stable knockdown of HOTAIR significantly suppressed tumor growth and sensitized cervical cancer to radiotherapy in vivo. 25547435 Lnc2Cancer
hepatocelluar carcinoma qPCR, Western blot etc. cell lines (HepG2, Bel7404, PLC5, Huh7 etc.) up-regulated interaction We found that Hotair was increased in HCC tissues compared to their adjacent non-tumor tissues and the normal liver tissues. Increased Hotair negatively regulated miR-218 expression in HCC, which might be mediated through an EZH2-targeting-miR-218-2 promoter regulatory axis. Further investigation revealed that Hotair knockdown dramatically inhibited cell viability and induced G1-phase arrest in vitro and suppressed tumorigenicity in vivo by promoting miR-218 expression. 26024833 Lnc2Cancer
bladder cancer qPCR, Western blot etc. bladder cancer tissue up-regulated interaction Our findings indicate that HOTAIR expression has prognostic value for bladder cancer progression, recurrence, and survival and suggest that HOTAIR plays active roles in modulating the cancer epigenome, becoming an interesting candidate as a target for cancer diagnosis and therapy. The observed HOTAIR regulation by EZH2 and the possibility of modulating EZH2 activity with specific inhibitors open new possible paths to be explored in bladder cancer therapy 26457124 Lnc2Cancer
head and neck squamous cell carcinoma qPCR, Western blot, Flow cytometry assay etc. cell lines (Tca8113, Tca8113P160, Tb3.1) differential expression interaction We employed a HOTAIR specific siRNA to deplete its expression in two human HNSCC cell lines, Tca8113 and Tscca. The flow cytometry (FCM) analysis showed that HOTAIR depletion induced tumor cell apoptosis in vitro. JC-1 probe examination showed that the mitochondrial membrane potential was changed significantly by HOTAIR blockage. Mitochondrial calcium uptake 1(MICU1) dependent cell death was induced by HOTAIR depletion. Protein expression analysis indicated that mitochondrial related cell death pathway (Bcl-2, BAX, Caspase-3, Cleaved Caspase-3, Cytochrome c) involved in HOTAIR dependent apoptosis process. Moreover, a Tscca derived xenograft tumor model was employed to further validate that injection of HOTAIR siRNA inhibited tumor growth. In summary, we suggested that HOTAIR inhibition could be developed as a new therapeutic in HNSCC treatments. 26592246 Lnc2Cancer
hepatocelluar carcinoma qPCR, Western blot, in vitro knockdown etc. cell line (Bel7402) up-regulated expression The HOTAIR gene was significantly overexpressed in HCC tissues compared with adjacent non-tumour tissues. Patients with high HOTAIR gene expression in their tumours had an increased risk of recurrence after hepatectomy. 22289527 LncRNADisease Lnc2Cancer
non-small cell lung cancer qPCR, Western blot, knockdown etc. NSCLC tissue, cell lines (A549, SPC-A1,NCI-H1975 etc.) up-regulated N/A HOTAIR was highly expressed both in NSCLC samples and cell lines compared with corresponding normal counterparts. HOTAIR upregulation was correlated with NSCLC advanced pathological stage and lymph-node metastasis. Moreover, patients with high levels of HOTAIR expression had a relatively poor prognosis. 24103700 Lnc2Cancer
lung adenocarcinoma qPCR, Western blot, knockdown etc. lung adenocarcinoma tissue, cell lines (A549/DDP, SPC-A1 etc.) up-regulated N/A In this study, we show that HOTAIR expression was significantly upregulated in cisplatin-resistant A549/DDP cells compared with in parental A549 cells. Knockdown of HOTAIR by RNA interference could resensitize the responses of A549/DDP cells to cisplatin both in vitro and in vivo. In contrast, overexpression of HOTAIR could decrease the sensitivity of A549 and SPC-A1 cells to cisplatin. 24155936 Lnc2Cancer
ovarian cancer qPCR, Western blot, knockdown etc. epithelial ovarian cancer tissue, cell lines (SKOV3, SKOV3.ip1 etc.) up-regulated expression Overexpression of long non-coding RNA HOTAIR predicts poor patient prognosis and promotes tumor metastasis in epithelial ovarian cancer. 24662839 LncRNADisease Lnc2Cancer
colon cancer qPCR, Western blot, knockdown etc. colon cancer tissue, cell lines (SW480, HT29) up-regulated expression Long?non-coding?RNA?HOTAIR is a powerful predictor of metastasis and poor prognosis and is associated with epithelial-mesenchymal transition in colon cancer. 24840737 LncRNADisease Lnc2Cancer
cervical cancer qPCR, Western blot, knockdown etc. cervical cancer tissue, cell lines (SiHa, HeLa, Caski) up-regulated expression The expression level of HOTAIR in cervical cancer tissues was higher than that in corresponding non-cancerous tissues. High HOTAIR expression correlated with lymph node metastasis, and reduced overall survival. Moreover, HOTAIR regulated the expression of vascular endothelial growth factor, matrix metalloproteinase-9 and epithelial-to-mesenchymal transition (EMT)-related genes, which are important for cell motility and metastasis. 25405331 Lnc2Cancer
ovarian cancer qPCR, Western blot, knockdown etc. cell lines (SKOV3) up-regulated interaction The results demonstrated that the HOTAIR expression in clinical EOC tissues and SKOV3 CD117(+)CD44(+)CSCs was higher than in SKOV3 tumor tissues and non-CD117(+)CD44(+)CSCs. 25792974 Lnc2Cancer
oral squamous cell carcinoma qPCR, Western blot, knockdown etc. OSCC tissue, cell lines (TSCCA, Tca8223 etc.) up-regulated N/A Long non-coding RNA HOTAIR promotes tumor cell invasion and metastasis by recruiting EZH2 and repressing E-cadherin in oral squamous cell carcinoma. 25901533 LncRNADisease Lnc2Cancer
colorectal cancer qPCR, Western blot, knockdown etc. CRC tissue, cell lines (FHC, CCL244, HCT116, SW480, LOVO) up-regulated expression Results showed that CRC patients had higher HOTAIR expression in tumor tissues compared with adjacent normal tissues. In vitro, downregulation of HOTAIR reduced proliferation, migration and invasiveness while enhanced apoptosis and radio-sensitivity of CRC cells. Taken together, our findings suggest that long non-coding RNA HOTAIR expression is closely associated with tumor invasion and radiosensitivity, indicating the potential role in diagnostics and therapeutics of CRC. 26549670 Lnc2Cancer
renal cell carcinoma qPCR, Western blot, knockdown, ChIP etc. cell lines (A-498, OS-RC-2) up-regulated N/A Suppressed expression of long non-coding RNA HOTAIR inhibits proliferation and tumourigenicity of renal carcinoma cells. 25149152 LncRNADisease Lnc2Cancer
ovarian cancer qPCR, Western blot, knockdown, MTT assay etc. ovarian cancer tissue, cell lines (A2780, 3AO, OVCAR3, SKOV3, HO-8910) up-regulated interaction HOTAIR overexpression promoted cell cycle progression (and thus cell proliferation) by activating the Wnt/β-Catenin signaling pathway. Likewise, knockdown of HOTAIR suppressed cell proliferation and arrested cell cycle at G1 phase via inhibition of Wnt/β-Catenin signaling. Moreover, the results of primary culture demonstrated that elevated HOTAIR expression correlated positively with chemoresistance in ovarian cancer. 26341496 Lnc2Cancer
acute myeloid leukemia qPCR, Western blot, knockdown, RIP etc. cell lines up-regulated interaction We report that HOTAIR expression was obviously increased in leukemic cell lines and primary AML blasts. Clinically, AML patients with higher HOTAIR predicted worse clinical outcome compared with those with lower HOTAIR. Importantly, HOTAIR knockdown by small hairpin RNA inhibited cell growth, induced apoptosis, and decreased number of colony formation. Finally, HOTAIR modulated c-KIT expression by competitively binding miR-193a. 25979172 Lnc2Cancer
gastric cancer qPCR, Western blot, Luciferase reporter assay, knockdown etc. gastric cancer tissue, cell lines (MGC-803, SGC-7901, BGC-823, AGS) up-regulated regulation Lnc RNA HOTAIR functions as a competing endogenous?RNA?to regulate HER2 expression by sponging miR-331-3p in gastric cancer. 24775712 LncRNADisease Lnc2Cancer
non-small cell lung cancer qPCR, Western blot, Luciferase reporter assay, knockdown etc. cell lines (A549, SK-MES-1) up-regulated interaction In the present study, we demonstrated that HOTAIR was upregulated by hypoxia in NSCLC cells. HOTAIR is a direct target of HIF-1αthrough interaction with putative HREs in the upstream region of HOTAIR in NSCLC cells. Furthermore, HIF-1α knockdown or inhibition could prevent HOTAIR upregulation under hypoxic conditions. Under hypoxic conditions, HOTAIR enhanced cancer cell proliferation, migration, and invasion. 26088446 Lnc2Cancer
liver cancer qPCR, Western blot, Luciferase reporter assay, RIP etc. liver cancer tissue, cell line (hLCSC) up-regulated interaction HOTAIR level was significantly higher in human hepatocarocinoma tissues and play tumorigenesis roles by downregulating SETD2 in liver cancer stem cells. HOTAIR may also mediate changes at an epigenetic level to affect gene expression and contribute to tumor aetiology. 26172293 Lnc2Cancer
bladder cancer qPCR, Western blot, Northern blot etc. cell lines(HCV29, 5637, T24, J82, SW780 ) up-regulated interaction We have identified cyclin J (CCNJ) gene, which is involved in cell cycle regulation, as a novel target for miR-205. Furthermore, a long non-coding RNA HOTAIR (HOX transcript antisense RNA) was observed to participate in the silencing of miR-205 in bladder cancer cells by breaking the balance of histone modification between H3K4me3 (histone H3 at lysine 4 methylation) and H3K27me3 on miR-205 promoter. 26469956 Lnc2Cancer
renal cancer qPCR, Western blot, RIP etc. cell lines (86-O, ACHN) up-regulated expression Long Non-coding RNA HOTAIR Is Targeted and Regulated by miR-141 in Human Cancer Cells. 24616104 LncRNADisease Lnc2Cancer
gastric cancer qPCR, Western bolt, in vitro knockdown etc. gastric cancer tissue, cell lines (GES-1, AGS, SGC-7901, MKN-45, HGC-27 etc.) up-regulated N/A The expression level of HOTAIR in cancer tissues was higher than that in adjacent noncancerous tissues. Expression level of HOTAIR was significantly correlated with lymph node metastasis and TNM stage. Furthermore, high expression level of HOTAIR was a predictor of poor over-all survival in GC patients. In vitro, inhibition of HOTAIR in GC cells could reduce invasiveness, as well as the expression of MMP1 and MMP3. In addition, suppression of HOTAIR could reverse EMT process. 23847441 Lnc2Cancer
glioma qRT-PCR, knock-down glioma U87 and U251 cell lines up-regulated expression knock-down of HOTAIR exerted tumor-suppressive function in glioma cells. Further, HOTAIR was confirmed to be the target of miR-326 and miR-326 mediated the tumor-suppressive effects of HOTAIR knock-down on glioma cell lines 26183397
pre-eclampsia qRT-PCR, knockdown, overexpression placentas from pre-eclampsia (PE) pregnant women up-regulated expression HOTAIR is probably involved in the onset of preeclampsia by regulating proliferation, invasion and apoptosis of trophoblast cells. 25807808
bladder transitional cell carcinoma quantitative real-time PCR, MTT assay, Dual-color flow cytometric method bladder transitional cell carcinoma (TCC) tissues and cell lines up-regulated expression HOTAIR over-expression promoted cell proliferation and inhibited chemosensitivity to doxorubicin and cell apoptosis induced by doxorubicin; silence of HOTAIR showed opposite regulative effects. lncRNA HOTAIR was an independent prognostic biomarker of overall survival in TCC patients and could regulate chemosensitivity to doxorubicin of human TCC cells. 26781446
breast cancer real-time polymerase chain reaction (PCR) using TaqMan assay 123 BC patients and 122 age-matched healthy controls N/A mutation TT genotype of HOTAIR rs12826786 C>T polymorphism was significantly related with multiple clinicopathological characteristics concerned with worse BC progression such as advanced TNM stage (III and IV), larger tumor size (T3 and T4), and distant metastasis (M1), as well as poor histological grade (III) (P < 0.05). 26577852
breast cancer RNA CISH etc. breast cancer tissue up-regulated expression HOTAIR, H19 and KCNQ1OT1 had significantly higher expression levels in IBC than NA, and HOTAIR and H19 were both expressed more strongly in IBC than in DCIS tissues. 26323944 Lnc2Cancer
infiltrating ductal carcinomas RNA-seq, qRT-PCR human mammary epithelial cells (non-transformed) and the breast cancer cell line MCF-7 up-regulated expression The lncRNA HOTAIR was significantly overexpressed in the HER2-enriched subgroup. 25296969
endometrial cancer RT-qPCR 156 consecutive, stage I-IV EC patients N/A N/A high HOTAIR expression correlated with shorter overall survival 26868332
breast cancer TaqMan allelic discrimination assay etc. blood differential expression expression We found that the CC genotype of HOTAIR rs920778 polymorphism significantly increased the risk of BC in both codominant and recessive inheritance genetic models. Our research also indicated an association between the CC genotype of HOTAIR rs920778 polymorphism and clinicopathologic features of tumor, including advanced tumor-node-metastasis (TNM) stage, larger tumor size, distant metastasis, and poor histological grade. CC genotype of HOTAIR rs920778 polymorphism might play important roles in genetic susceptibility to BC development and aggressiveness in a Turkish population. 25586347 Lnc2Cancer
gastric cancer TaqMan RT-PCR gastric cancer samples N/A mutation A naturally occurring functional single nucleotide polymorphism (SNP) rs920,778 (C→T) in the intronic enhancer of HOTAIR gene has been demonstrated to affect HOTAIR expression and cancer susceptibility. Our results demonstrate that the HOTAIR rs920778 polymorphism has not been in any major role in genetic susceptibility to gastric carcinogenesis, at least in the population studied here. 25980897
diffuse large B-cell lymphoma The reverse transcription‑polymerase chain reaction & Western blotting N/A up-regulated N/A HOTAIR was significantly correlated with tumor size, clinical stage, B symptoms and International Prognostic Index scores 27122348
colorectal cancer the tumorigenicity of CD133(+)-shHOTAIR were evaluated by the MTT CD133(+)CSCs cell down-regulated N/A down-regulating the HOTAIR expression in CD133(+)CSCs could serve as a potential anti-cancer regimen to inhibit the invasiveness and metastasis of CRC CSCs 27069543
 


Function (not disease relevant)
Methods Sample/condition Expression pattern Dysfunction type Description PMID Source
Illumina microarrays and confirmed by RT-PCR Abdominal and gluteal adipose tissue aspirates obtained from 35 subjects N/A interaction Ectopic expression of HOTAIR in abdominal preadipocytes produced an increase in differentiation as reflected by a higher percentage of differentiated cells, and increased expression of key adipogenic genes including PPARγ and LPL 24862299
knockdown urothelial bladder cancer (UBC), urine (Ues) up-regulated interaction Knockdown of HOTAIR in UBC cell lines reduces in vitro migration and invasion. 26800519
knock-down human cardiomyocytes N/A expression up-regulation of HOTAIR could suppress the expression of CaV1.2 in human cardiomyocytes. However, HOTAIR knock-down promoted CaV1.2 expression in human cardiomyocytes. enforced expression of CaV1.2 increased the calcium level in cardiomyocytes overexpressing HOTAIR. These results for the first time demonstrate that HOTAIR inhibited the intracellular Ca2+ via regulation of CaV1.2 in human cardiomyocytes. 26255135
knockdown, Luciferase assays 80 clinical colon cancer tissues up-regulated interaction N/A 26962687
LncRNA microarray Dermal papilla (DP) cells up-regulated expression RP11-766N7.3, H19 and HOTAIR are specific lncRNAs that were aberrantly expressed in DP cells and played an important role in regulating Wnt signaling 25285630
N/A breast cancer cells N/A interaction Silencing of HBXIP, Hotair, or LSD1 was sufficient to block c-Myc-enhanced cancer cell growth in vitro and in vivo. Taken together, our results support a model in which the HBXIP/Hotair/LSD1 complex serves as a critical effector of c-Myc in activating transcription of its target genes, illuminating long-standing questions on how c-Myc drives carcinogenesis. 26719542
N/A N/A N/A N/A serve as scaffolds by providing binding surface 20616235
RT-PCR,Conventional ChIP and ChIP-chip ,In situ hybridization,RNA interference N/A N/A N/A We identified a 2.2 kilobase ncRNA residing in the HOXC locus, termed HOTAIR, which represses transcription in trans across 40 kilobases of the HOXD locus. HOTAIR interacts with Polycomb Repressive Complex 2 (PRC2) and is required for PRC2 occupancy and histone H3 lysine-27 trimethylation of HOXD locus. Thus, transcription of ncRNA may demarcate chromosomal domains of gene silencing at a distance; 17604720
 


Interaction
Interaction target Level of interaction Type of interaction Description PMID Source
HOXD RNA-DNA regulation HOTAIR is transcribed at the intersection of opposing chromatin domains in the HOXC locus, but targets Polycomb Repressive Complex 2 (PRC2) to represses transcription in trans across 40 kilobases of the HOXD locus. 17604720 LncRNADisease
PRC2 RNA-Protein binding HOTAIR is transcribed at the intersection of opposing chromatin domains in the HOXC locus, but targets Polycomb Repressive Complex 2 (PRC2) to silence 40 kilobases of HOXD. 17604720 LncRNADisease
HOXD RNA-DNA regulation HOTAIR represses transcription in trans across 40 kilobases of the HOXD locus. 17604720 LncRNADisease
HOXD locus,Polycomb Repressive Complex 2 (PRC2) RNA-Protein regulation HOTAIR, which represses transcription in trans across 40 kilobases of the HOXD locus. HOTAIR interacts with Polycomb Repressive Complex 2 (PRC2) and is required for PRC2 occupancy and histone H3 lysine-27 trimethylation of HOXD locus. 17604720
HOXD RNA-DNA regulation HOTAIR represses the expression in trans of genes in the HOXD cluster. Depletion of HOTAIR by siRNA resulted in the activation of HOXD genes in trans. 19015002 LncRNADisease
Ezh2 RNA-Protein binding HOTAIR RNA plays a critical role in establishing H3K27me3 enriched chromosomal domains through interaction with PRC2 complex members such as Suz12 and Ezh2. 19015002 LncRNADisease
Suz12 RNA-Protein binding HOTAIR RNA plays a critical role in establishing H3K27me3 enriched chromosomal domains through interaction with PRC2 complex members such as Suz12 and Ezh2. 19015002 LncRNADisease
HOXD RNA-RNA co-expression HOTAIR showed negative association with HOXD genes. 19182780 LncRNADisease
PRC2 RNA-Protein binding HOTAIR coprecipitated with PRC2 in both HeLa and hFFs, but not in hLFs. 19571010 LncRNADisease
PRC2 RNA-Protein binding HOTAIR expression may be involved in human cancer by promoting genomic relocalization of Polycomb complex and H3K27 trimethylation. 20393566 LncRNADisease
H3 RNA-Protein regulation HOTAIR expression may be involved in human cancer by promoting genomic relocalization of Polycomb complex and H3K27 trimethylation. 20393566 LncRNADisease
H3 RNA-Protein regulation HOTAIR expression may be involved in human cancer by promoting genomic relocalization of Polycomb complex and H3K27 trimethylation. 20393566 LncRNADisease
LSD1 RNA-Protein binding A 3' domain of HOTAIR binds the LSD1/CoREST/REST complex. 20616235 LncRNADisease
PRC2 RNA-Protein binding A 5'domain of HOTAIR binds polycomb repressive complex 2 (PRC2). 20616235 LncRNADisease
LSD1 RNA-Protein binding Immunoprecipitation (IP) of LSD1 retrieved endogenous HOTAIR with comparable enrichment to that of PRC2 IP. It was hypothesized that HOTAIR may also bind the LSD1-CoREST complex. 20616235 LncRNADisease
HOXD RNA-DNA regulation The lincRNA HOTAIR is transcribed from the HOXC locus and targets Polycomb Repressive Complex 2 (PRC2, comprised of H3K27 methylase EZH2, SUZ12, and EED) to silence HOXD and select genes on other chromosomes. 20616235 LncRNADisease
PRC2 RNA-Protein binding The lincRNA HOTAIR is transcribed from the HOXC locus and targets Polycomb Repressive Complex 2 (PRC2, comprised of H3K27 methylase EZH2, SUZ12, and EED) to silence HOXD and select genes on other chromosomes. 20616235 LncRNADisease
PRC2 RNA-DNA regulation binding surface assemble select histone modification enzymes thereby specifying the pattern of histone modifications on target genes 20616235
HOXD RNA-Protein regulation The lncRNA HOTAIR is a putative direct positive regulator of the Polycomb repressor complex, and is consequently an indirect negative regulator of homeobox-D transcription factors. 20951849 LncRNADisease
PRC2 RNA-Protein regulation The lncRNA HOTAIR is a putative direct positive regulator of the Polycomb repressor complex. 20951849 LncRNADisease
Ezh2 RNA-Protein binding Ezh2 interacts with HOTAIR and Xist. 21123648 LncRNADisease
CoREST RNA-Protein binding Epigenetically silences gene expression at many loci by recruitment of LSD1/CoREST/REST and PRC2 repressive chromatin modifying complexes. Oncogene: promotes tumour metastasis. 21256239 LncRNADisease
LSD1 RNA-Protein binding Epigenetically silences gene expression at many loci by recruitment of LSD1/CoREST/REST and PRC2 repressive chromatin modifying complexes. Oncogene: promotes tumour metastasis. 21256239 LncRNADisease
PRC2 RNA-Protein binding Epigenetically silences gene expression at many loci by recruitment of LSD1/CoREST/REST and PRC2 repressive chromatin modifying complexes. Oncogene: promotes tumour metastasis. 21256239 LncRNADisease
REST RNA-Protein binding Epigenetically silences gene expression at many loci by recruitment of LSD1/CoREST/REST and PRC2 repressive chromatin modifying complexes. Oncogene: promotes tumour metastasis. 21256239 LncRNADisease
PRC2 RNA-Protein binding CDK1 and CDK2 phosphorylate EZH2 at threonine 350 (T350) and that T350 phosphorylation is important for the binding of EZH2 to PRC2 recruiters, such as noncoding RNAs (ncRNAs) HOTAIR and XIST. 21278485 LncRNADisease
LSD1 RNA-Protein binding Analysis of HOTAIR revealed that a 5' end domain binds PRC2 and a 3' end domain binds an LSD1 (H3K4me2 demethylase) containing complex. 21496640 LncRNADisease
PRC2 RNA-Protein binding Analysis of HOTAIR revealed that a 5' end domain binds PRC2 and a 3' end domain binds an LSD1 (H3K4me2 demethylase) containing complex. 21496640 LncRNADisease
HOXD RNA-DNA regulation HOTAIR, a paradigm of this new class of RNAs, is localized within the human HOXC gene cluster and was shown,in human cells, to regulate HOXD genes in trans via the recruitment of Polycomb Repressive Complex 2 (PRC2), followed by the trimethylation of lysine 27 of histone H3. 21637793 LncRNADisease
PRC2 RNA-Protein binding HOTAIR, a paradigm of this new class of RNAs, is localized within the human HOXC gene cluster and was shown,in human cells, to regulate HOXD genes in trans via the recruitment of Polycomb Repressive Complex 2 (PRC2), followed by the trimethylation of lysine 27 of histone H3. 21637793 LncRNADisease
PRC2 RNA-Protein binding HOTAIR, a paradigm of this new class of RNAs, is localized within the human HOXC gene cluster and was shown,in human cells, to regulate HOXD genes in trans via the recruitment of Polycomb Repressive Complex 2 (PRC2), followed by the trimethylation of lysine 27 of histone H3. 21637793 LncRNADisease
H3 RNA-Protein regulation HOTAIR, a paradigm of this new class of RNAs, is localized within the human HOXC gene cluster and was shown,in human cells, to regulate HOXD genes in trans via the recruitment of Polycomb Repressive Complex 2 (PRC2), followed by the trimethylation of lysine 27 of histone H3. 21637793 LncRNADisease
Hox RNA-DNA regulation HOTAIR is transcribed from within specific Hox gene clusters but regulates the expression of Hox genes located on different chromosomes. 21936910 LncRNADisease
EZH2 RNA-Protein binding HOTAIR, Kcnq1ot and Xist all mediate their effects by interacting with the Polycomb-repressive complex 2 (PRC2) component Ezh2 (enhancer of zeste homolog 2 (Drosophila)) and modulating histone methylation. 21936910 LncRNADisease
β-catenin RNA-Protein regulation β-catenin is a stretch responsive signaling pathway that represses HOTAIR 24788418
PPARγ and LPL RNA-DNA regulation Ectopic expression of HOTAIR in abdominal preadipocytes produced an increase in differentiation as reflected by a higher percentage of differentiated cells, and increased expression of key adipogenic genes including PPARγ and LPL 24862299
Wnt inhibitory factor 1 RNA-Protein regulation A significant inverse correlation between HOTAIR and WIF-1 expression was demonstrated in Ta/T1 BC tissues. 25030736
Cisplatin and paclitaxel N/A regulation Cisplatin and paclitaxel have target function on significant lncRNAs in LSCC, which presents novel molecular targets to cure LSCC patients and also leads an orientation for developing new drugs. 25257554
SiRNA RNA-RNA regulation SiRNA knockdown of HOTAIR in GC cells significantly inhibited cell proliferation 25280565
histone H3 lysine27 RNA-Protein binding MCF-7-TNR cells exhibited reduced suppressive histone H3 lysine27 trimethylation on the HOTAIR promoter. 25328122
endothelial growth factor, matrix metalloproteinase-9 and epithelial-to-mesenchymal transition (EMT)-related genes RNA-DNA regulation HOTAIR regulated the expression of vascular endothelial growth factor, matrix metalloproteinase-9 and epithelial-to-mesenchymal transition (EMT)-related genes 25405331
protein coding gene RNA-DNA regulation GO analyses of most significantly correlated coding genes of HOTAIR and lncRNA-422 showed that these two lncRNAs may participate in carcinogenesis by regulating protein coding genes involved in special biological process relevant to cancer. 25456707
p21 RNA-DNA regulation Knockdown of HOTAIR upregulated p21. 25547435
shHOTAIR RNA-RNA regulation Our findings demonstrated the shHOTAIR-mediated down-regulation of the HOTAIR expression in CD117(+)CD44(+) CSCs can be a promising new opportunity for future clinical trials. 25792974
β-catenin DNA-Protein regulation We show that expression of HOTAIR is regulated by β-catenin through a LEF1/TCF4-binding site. The dual treatment blocks nuclear expression of β-catenin and prevents its recruitment to the HOTAIR promoter. 25883211
c-KIT,miR-193a RNA-DNA binding HOTAIR modulated c-KIT expression by competitively binding miR-193a. 25979172
miR-218 RNA-RNA regulation Hotair negatively regulated miR-218 expression in HCC, which might be mediated through an EZH2-targeting-miR-218-2 promoter regulatory axis. 26024833
HIF-1α DNA-Protein binding The function of the hypoxia-inducible factor-1α (HIF-1α) binding site to hypoxia-responsive elements (HREs) in the HOTAIR promoter region. HOTAIR is a direct target of HIF-1α through interaction with putative HREs in the upstream region of HOTAIR in NSCLC cells. 26088446
E7 RNA-Protein co-expression Expression of HOTAIR and PRC2-complex members (EZH2 and SUZ12), showed significant positive correlation with E7 expression in CaCx cases and E7 transfected C33A cell line, suggestive of interplay between E7 and HOTAIR. 26152361
SETD2 RNA-DNA regulation HOTAIR promotes tumorigenesis via downregulating SETD2 in liver cancer stem cells. 26172293
miR-326 RNA-RNA regulation HOTAIR was confirmed to be the target of miR-326 . knock-down of HOTAIR up-regulated miR-326 expression. 26183397
miR-152 RNA-RNA regulation Long non-coding RNA HOTAIR promotes HLA-G expression via inhibiting miR-152 in gastric cancer cells. 26187665
CaV1.2 RNA-Protein regulation In the present study, we demonstrated that up-regulation of HOTAIR could suppress the expression of CaV1.2 in human cardiomyocytes. 26255135
Wnt/β-catenin pathway N/A regulation HOTAIR promotes the initiation and chemoresistance of ovarian cancer by activating wnt/β-catenin signaling. 26341496
ER RNA-Protein regulation The long non-coding RNA HOTAIR is directly repressed by ER and its upregulation promotes ligand-independent ER activities and contributes to tamoxifen resistance. 26364613
AR RNA-Protein binding HOTAIR binds to the AR protein to block its interaction with the E3 ubiquitin ligase MDM2, thereby preventing AR ubiquitination and protein degradation. 26411689
EZH2 RNA-Protein regulation A significant correlation between EZH2 and HOTAIR expression levels. EZH2 regulates HOTAIR expression. 26457124
microRNA-205 RNA-RNA regulation A long non-coding RNA HOTAIR (HOX transcript antisense RNA) was observed to participate in the silencing of miR-205 in bladder cancer cells by breaking the balance of histone modification between H3K4me3 (histone H3 at lysine 4 methylation) and H3K27me3 on miR-205 promoter. 26469956
cell death pathway N/A regulation Protein expression analysis indicated that mitochondrial related cell death pathway (Bcl-2, BAX, Caspase-3, Cleaved Caspase-3, Cytochrome c) involved in HOTAIR dependent apoptosis process. 26592246
LSH RNA-Protein regulation HOTAIR is associated with LSH, and this association linked with the binding of LSH in the promoter of FOXA1, not FOXA2. 26658322
FOXA1, FOXA2 RNA-Protein regulation HOTAIR regulates the ratio of FOXA1 to FOXA2 and migration and invasion. 26658322
VEGFA and Ang2 RNA-Protein regulation Hotair promoted angiogenesis through directly activating the transcription of angiogenic factor VEGFA as well as through GRP78-mediated upregulation of VEGFA and Ang2 expression. 26717040
c-Myc RNA-DNA binding HBXIP interacted directly with c-Myc through the leucine zippers and recruited the lncRNA Hotair along with the histone demethylase LSD1, for which Hotair serves as a scaffold. 26719542
epithelial-to-mesenchyme transition (EMT) genes RNA-DNA regulation Loss of HOTAIR expression in UBC cell lines alters expression of epithelial-to-mesenchyme transition (EMT) genes including SNAI1, TWIST1, ZEB1, ZO1, MMP1 LAMB3, and LAMC2. 26800519
TNF-α, NF-κB RNA-Protein regulation HOTAIR upregulation in cardiomyocytes of LPS-induced sepsis mice promoted TNF-α production in the circulation by activating NF-κB, involving the phosphorylation of NF-κB p65 subunit. 26806307
miR-125a-5p RNA-RNA regulation microRNA miR-125a-5p decreases and releases caspase 2 to promote cancer cell apoptosis after HOTAIR knockdown. 26962687