| RT-PCR |
vernalization |
down-regulated |
N/A |
COLDAIR, an intronic long ncRNA of the FLC. COLDAIR is required for the vernalization-mediated epigenetic repression of FLC. COLDAIR physically associates with a component of PRC2 and targets PRC2 to FLC. Our results show that COLDAIR is required for establishing stable repressive chromatin at FLC through its interaction with PRC2. COLDAIR is required for proper repression of FLC during the course of vernalization. COLDAIR is necessary for the recruitment of CLF to FLC chromatin. (Heo et al., 2011) COLDAIR lncRNAs transcribed from the first intron of FLC are also transiently induced by the cold. The CLF subunit of PHD–PRC2 associates with COLDAIR, leading to the enrichment of this complex on FLC chromatin in the cold. A partial deletion of the COLDAIR promoter or knockdown of COLDAIR expression compromises FLC silencing. Thus, COLDAIR is required for FLC silencing by vernalization. (He et al., 2012) COLDAIR, provided the evidence that the lncRNA plays a crucial role in the regulation of flowering time by recruiting a chromatin remodeling complex to the proper target, FLC, for stable silencing by vernalization. COLDAIR, is transcribed from the first intron of FLC and its expression is increased by cold. COLDAIR physically interacts with PRC2 and is required for the enrichment of PRC2 at FLC chromatin by vernalization. Reduced COLDAIR expression by RNAi compromises the vernalization response. The interaction between COLDAIR and PRC2 increases transiently only during the cold exposure but COLDAIR is necessary for the stable maintenance of silenced FLC after cold. (Kim et al., 2012) COLDAIR play a role in the intragenerational repression of FLC. COLDAIR is an lncRNA that is expressed in the sense direction from the first intron of FLC. COLDAIR is induced during cold exposure and associates with PRC2. Its knockdown leads to a decrease in epigenetic silencing, less H3K27me3, implicating it as a required component in the recruitment of PRC2. (Jones et al., 2014) A sense intronic lncRNA named COLDAIR, participate in different phases of the cold-induced repression and the stable silencing of FLC by PRC2. Cold-induced COOLAIR-like and COLDAIR-like lncRNAs could be involved, respectively, in the initial downregulation of DAM genes and the function of PRC2 complexes. (Ríos et al., 2014) In Arabidopsis the COLDAIR lncRNA-triggered chromatin silencing of a central floral repressor known as FLC, involves H3K27me3, but not cytosine methylation. In response to a prolonged cold exposure, COLDAIR expression is induced from the first intron of FLC, and subsequently, this lncRNA recruits a PRC2-like complex to FLC chromatin to deposit H3K27me3, leading to FLC silencing. (He et al., 2013) A long intronic noncoding RNA, named COLD ASSISTED INTRONIC NONCODING RNA (COLDAIR), was shown to be required for the vernalization-mediated epigenetic repression of FLC. COLDAIR physically associates with CLF, suggesting that COLDAIR establishes stable repressive chromatin at FLC through recruiting PRC2 in H3K27me3 deposition. (Yao et al., 2011) In Arabidopsis, the floral repressor FLOWERING LOCUS C (FLC), a master regulator of flowering, is stably suppressed by prolonged exposure to cold, which promotes the enrichment of tri-methylated histone H3K27me3 at FLC locus. This process is mediated by the interaction of a long intronic noncoding RNA (named COLDAIR) to the FLC locus via recruitment of PRC2. (Pignatta et al., 2012) A long intronic noncoding RNA (COLD ASSISTED INTRONIC NONCODING RNA, COLDAIR) is also required for the stable repression of FLC after cold. COLDAIR is transcribed from the first intron of FLC during vernalization and it targets PRC2 to the FLC chromatin. (Justyna et al., 2012) The COLDAIR sense transcript is produced from within FLC intron 1, and plays a role in FLC silencing via recruitment of Polycomb repressing complex 2 (PRC2) to FLC during vernalization. The establishment of H3K27 trimethylation to silence FLC in vernalization requires COLDAIR. (Shin et al., 2014) long noncoding RNA called COLDAIR shown to be important in regulating vernalization responses in Arabidopsis. (Moghe et al., 2012) In mediating transcriptional regulation, histone modification can also involve ncRNAs such as COLDAIR, a cold-inducible long ncRNA that increases H3K27me3 marks at chromatin of the floral repressor FLC through recruitment of polycomb repressive complex 2. (Zhang et al., 2013) Two noncoding RNAs have now been implicated in vernalization induced recruitment of PHD-PRC2. One of these, termed COLDAIR, is a long (about 1 kb) noncoding sense RNA produced from sequences within the large first intron of FLC. Expression of COLDAIR is cold-induced and several observations suggest that it is functionally important for FLC silencing. In particular, transgenes with deletions that removed part or all of the COLDAIR gene showed fairly normal downregulation during cold treatments but regained activity when plants were returned to warm conditions. (Müller et al., 2011) |
21127216, 22658650, 22078062, 24808013, 24917873, 23000433, 25211139, 23132786, 23838953, 21876724 |
PLNlncRbase
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