| RT-PCR/Northern blot |
flower buds |
up-regulated |
N/A |
A 1.8-kb transcript was observed not only in male flower buds but also in female flower buds. The hybridization signal of CCLS96.1 in the Northern blot analysis was so week that semi-quantitative RT-PCR was performed to analyze the expression patterns of CCLS96.1. CCLS96.1 was expressed also in male and female leaves.In the male plant, the expression of CCLS96.1 in flower buds was 2.67-to 3.42-fold higher than that in leaves. In contrast, in the female plant, the expression of CCLS96.1 in flower buds was 0.06-to 0.19-fold less than that in leaves. This suggests that the CCLS96.1 homologs play some role in the male flower bud. CCLS96.1 was also expressed in both male and female leaves. CCLS96.1 homologs are expressed in leaves and buds. The CCLS96.1 homologs play some role in the male flower bud. (Sugiyama et al., 2003) CCLS96.1 expressed in both genders in leaves and flowers, yet it is expressed 3-fold higher in male flowers than in female flowers, and 4-8-fold higher in female leaves than in male leaves suggestive that CCLS96.1 plays a role in male flowers and perhaps female leaves, a mode of action has yet to be ascertained. (Rymarquis et al., 2008) |
14686583, 18448381 |
PLNlncRbase
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