LncRNA Information
ID EL0040 Name AFAP1-AS1 Aliases AFAP1-AS; AFAP1AS
Species Homo sapiens Chromosome 4 Start site 7754090
End site 7778928 Chain plus Exon NO. 2
Assembly Ensembl Release 89 Class antisense NCBI accession NR_026892
Ensembl ENSG00000272620 Sequence


Disease
Disease Method Sample Expression pattern Dysfunction type Description PMID Source
nasopharyngeal carcinoma microarray, qPCR, Western blot, knockdown, ISH etc. NPC tissue, cell lines (5-8F, HNE2, HK-1) up-regulated interaction AFAP1-AS1 expression was upregulated in NPC and associated with NPC metastasis and poor prognosis. In vitro experiments demonstrated that AFAP1-AS1 knockdown significantly inhibited the NPC cell migration and invasive capability. AFAP1-AS1 knockdown also increased AFAP1 protein expression. Proteomic and bioinformatics analyses suggested that AFAP1-AS1 affected the expression of several small GTPase family members and molecules in the actin cytokeratin signaling pathway. AFAP1-AS1 promoted cancer cell metastasis via regulation of actin filament integrity. 26246469 Lnc2Cancer
Barrett's esophagus N/A N/A N/A expression Hypomethylation of Noncoding DNA Regions and Overexpression of the Long Noncoding RNA, AFAP1-AS1, in Barrett's Esophagus and Esophageal Adenocarcinoma. 23333711 LncRNADisease Lnc2Cancer
esophageal squamous cell carcinoma overexpression, Kaplan-Meier survival analysis cisplatin-resistant and parental ESCC cell lines up-regulated expression High expression of AFAP1-AS1 could serve as a potential biomarker to predict tumor response and survival. Determination of this lncRNA expression might be useful for selection ESCC patients for dCRT. 26756568
esophageal adenocarcinoma qPCR etc. EAC tissue, cell lines (OE-33, SK-GT-4, FLO-1, HEEpiC etc.) up-regulated expression Hypomethylation of Noncoding DNA Regions and Overexpression of the Long Noncoding RNA, AFAP1-AS1, in Barrett's Esophagus and Esophageal Adenocarcinoma. 23333711 LncRNADisease Lnc2Cancer
pancreatic ductal adenocarcinoma qPCR etc. blood, cell lines (Panc1, MIAPaCa-2, Capan2, SW1990 etc.) up-regulated expression Microarray analysis revealed that up-regulation of AFAP1-AS1 expression in PDAC tissues compared with normal adjacent tissues, which was confirmed by RT-qPCR in 69/90 cases (76.7%). Its overexpression was associated with lymph node metastasis, perineural invasion, and poor survival. AFAP1-AS1 is a potential novel prognostic marker to predict the clinical outcome of PDAC patients after surgery and may be a rational target for therapy. 25925763 Lnc2Cancer
non-small cell lung cancer qPCR etc. non-small-cell lung cancer tissue up-regulated expression Results showed that patients with high LncRNA AFAP1-AS1 expression lived shorter than those with low LncRNA AFAP1-AS1 expression (Log rank test, P<0.001). Besides, the prognostic value of LncRNA AFAP1-AS1 as well as the clinical features was assessed by Cox regression analysis. The outcome revealed that LncRNA AFAP1-AS1 was closely related to the prognosis of NSCLC 26463625 Lnc2Cancer
hepatocelluar carcinoma real-time PCR; MTT assay 78 HCC tissues up-regulated N/A AFAP1-AS1 was significantly correlated with pathological staging 26892468
lung cancer RNA-seq, qPCR, Western blot etc. cell line (A549 ) up-regulated interaction AFAP1-AS1 was the most significantly upregulated in lung cancer and associated with poor prognosis. AFAP1-AS1 knockdown significantly inhibited the cell invasive and migration capability in lung cancer cells. AFAP1-AS1 knockdown also increased the expression of its antisense protein coding gene, actin filament associated protein 1 (AFAP1), and affected the expression levels of several small GTPase family members and molecules in the actin cytokeratin signaling pathway, which suggested that AFAP1-AS1 promoted cancer cell metastasis via regulation of actin filament integrity. 26245991 Lnc2Cancer
 


Function (not disease relevant)
Methods Sample/condition Expression pattern Dysfunction type Description PMID Source
N/A hepatocellular carcinoma (HCC) tissues up-regulated expression High AFAP1-AS1 expression was associated with tumor size, TNM stage, vascular invasion, and poor prognosis. Silencing of AFAP1-AS1 significantly reduced cell proliferation, clonal growth, cell migration, and invasion and increased apoptosis in vitro. AFAP1-AS1 silencing markedly reduced tumor growth in a murine allograft model in vivo. 26803513
 


Interaction
Interaction target Level of interaction Type of interaction Description PMID Source
several small GTPase family members RNA-Protein regulation AFAP1-AS1 knockdown affected the expression levels of several small GTPase family members and molecules in the actin cytokeratin signaling pathway. 26245991
actin filament associated protein 1 (AFAP1) RNA-Protein regulation AFAP1-AS1 knockdown also increased the expression of its antisense protein coding gene, actin filament associated protein 1 (AFAP1). 26245991
actin filament associated protein 1 (AFAP1) RNA-Protein regulation AFAP1-AS1 knockdown also increased AFAP1 protein expression. 26246469