LncRNA Information
ID	EL0945	Name	MtENOD40-1	Aliases	N/A
Species	Medicago truncatula	Chromosome	N/A	Start site	N/A
End site	N/A	Chain	N/A	Exon NO.	N/A
Assembly	N/A	Class	N/A	NCBI accession	N/A
Ensembl	N/A	Sequence	N/A

Function (not disease relevant)
Methods	Sample/condition	Expression pattern	Dysfunction type	Description	PMID	Source
RT-PCR	Nodule formation	down-regulated	N/A	In M.truncatula, knock-down of MtENOD40-1 expression led to a 50% reduction in the number of nodules. To reduce MtENOD40-1 and MtENOD40-2 gene expression, Agrobacterium rhizogenes-mediated RNAi was applied in M.truncatula hairy roots. To this end, one vector (pRRsil401) was designed that is expected to lead to a reduction in expression of MtENOD40-1, and a second vector (pRRsil402) that is expected to lead to a reduction in MtENOD40-2 expression. To knock-down transcription of both genes simultaneously, a third vector (pRRsil4012) was used. MtENOD40-1 as well as MtENOD40-2 is involved in nodule initiation. On roots,in which the expression of both MtENOD40-1 and MtENOD40-2 is reduced, the average number of nodules per root is 1.5 (75% reduction). Thus knocking down of the expression of both genes has an additive effect, suggesting that MtENOD40 acts in a dose-dependent manner.	17452749	PLNlncRbase