| RT-PCR |
normal pollen development |
up-regulated |
N/A |
Long-day-specific male-fertility-associated RNA (LDMAR), regulates PSMS in rice. We found that sufficient amount of the LDMAR transcript is required for normal pollen development of plants grown under long-day conditions. LDMAR is required for male fertility under long day conditions. The spontaneous G→C mutation causing a SNP between 58N and 58S altered the RNA secondary structure of this region. This alteration eventually brought about heritable increased methylation in the promoter region of LDMAR, thus reducing the level of transcription specifically under long-day conditions. Such a reduced level of LDMAR results in premature PCD in anther development under long days, thus causing male sterility. (Ding et al., 2012) Photoperiod-sensitive male sterility (PSMS) is a valuable germplasm for hybrid rice breeding. Recently, we cloned pms3, a locus controlling PSMS, which encodes a long non-coding RNA called LDMAR required for normal male fertility of the rice plant under long-day conditions.Increased methylation in the promoter of LDMAR in the PSMS rice (Nongken 58S) relative to the wild-type (Nongken 58) reduced expression of LDMAR leading to male sterility under long-day conditions.all in all, reduced the expression of this lncRNA, resulting in male sterility under long-day conditions. LDMAR required for normal male fertility of the rice plant under long-day conditions. Reduction of LDMAR in Nongken 58S changed the critical day length for fertility recovery and delayed the fertility recovery under short-day conditions. This result added to our understanding of the molecular mechanism for PSMS. (Ding et al., 2012) A long non-coding RNA (lncRNA) named LDMAR. A sufficient amount of LDMAR is required for male fertility under long-day conditions. A spontaneous G-C mutation causing a SNP between NK58 and NK58S, eventually brings about heritable increased methylation in the promoter region of LDMAR, which reduces the level of LDMAR expression. This then results in premature programmed cell death (PCD) in anther development under long days, and hence male sterility. In addition, RNA-dependent DNA methylation (RdDM) is involved in the regulation of PGMS. Promoter siRNA of LDMAR derived from AK11270 is associated with the DNA methylation level of LDMAR, which reduces the expression level of LDMAR, and therefore male sterility in Nonken 58S under long-day conditions. (Wang et al., 2013) |
22308482, 23024213, 23596452 |
PLNlncRbase
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