| Northern blot/Southern blot |
Nodule formation |
down-regulated |
N/A |
The alb1 is a symbiotic mutant of lotus japonicus that form empty nodules, mature alb1 nodules exhibied very weak or no expression of enod40 in the peripheral cells of the undeveloped nodule vascular bundle. ENOD40 may play a role in the differentiation of nodule vascular bundles. Expression of enod40 in alb1 nodules is restricted to early stages of nodule initiation. Nodule initiation on alb1 root is accompanied by the expression of enod40 in the same spatial pattern as and at a level comparable to, those in the wild type nodules. (Imaizumi-Anraku et al., 2000) Knock-down of ENOD40 leads to severe suppression of nodule primordium initiation but does not affect early bacterial infection events, thus clearly indicating that ENOD40 is required for nodule primordium formation as well as for subsequent nodule rganogenesis. knockdown of two ENOD40s in L.japonicus caused strong inhibition of nodulation. (Kumagai et al., 2006) In situ localization of LjENOD40 genes transcripts in Lotus japonicus(Gifu B-129) root nodules, young seed pods, and embryos. ENOD40, an early nodulin gene, has been postulated to play a significant role in legume root nodule ontogenesis. High levels of ENOD40 gene transcripts were found in nonsymbiotic tissues such as stems, fully developed flowers, green seed pods, and hypocotyls. lower level of transcripts was observed in leaves, roots, and cotyledons. In mature nodules, transcripts of both ENOD40 genes accumulate in the nodule vascular system; in young seed pods strong signal is observed in the ovule, particularly in the phloem and epithelium, as well as in globular stage embryos. ENOD40 genes are indirectly triggered by Nod factors via an effect on the levels of common plant hormones such as auxin. The expression of ENOD40 in developing embryos, as well as in ovule tissues, of L.japonicus adds further proof to the suggestion that the ENOD40 gene is a plant gene involved in organogenesis. (Flemetakis et al., 2000) |
11129043, 16816411, 10975655 |
PLNlncRbase
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