LncRNA Information
ID EL1052 Name PCAT1 Aliases PCA1; PCAT-1
Species Homo sapiens Chromosome 8 Start site 126847055
End site 127021014 Chain plus Exon NO. 2
Assembly Ensembl Release 89 Class lincRNA NCBI accession NR_045262
Ensembl ENSG00000253438 Sequence


Disease
Disease Method Sample Expression pattern Dysfunction type Description PMID Source
prostate cancer microarray, qPCR, Western blot, knockdown, Luciferase reporter assay etc. prostate tissue, cell lines (Du145-derived, RWPE-derived, LNCAP-derived, PC3-derived etc.) up-regulated regulation PCAT1, a long noncoding RNA, regulates BRCA2 and controls homologous recombination in cancer. 24473064 LncRNADisease Lnc2Cancer
prostate cancer microarray, RNA-seq, qPCR, Northern bolt, knockdown etc. prostate cancer tissue, cell lines (LNCaP, CWR22Rv1, PC3 etc.) up-regulated N/A Interestingly, nine out of these thirteen known cancer-related lncRNA showed significantly differential expression between tumor and normal prostate samples. Several lncRNA such as NEAT1, DANCR, HOTTIP, PRINS, and EGOT that have established functions in forming nuclear speckles, in development or in autoimmune disease, but were not previously known to be related to cancer, showed differential expression between tumor and normal prostate samples, suggesting their potential function in prostate cancer. 23728290 Lnc2Cancer
prostate cancer N/A N/A N/A regulation They described PCAT1,a novel PCa lincRNA on 8q24, in the locality of well-characterized PCa risk-related SNPs and the c-MYC oncogene. 24146262 LncRNADisease
prostate cancer N/A N/A N/A expression Putative marker and oncogene 24373479 LncRNADisease
prostate cancer N/A N/A N/A regulation The prostate cancer-associated ncRNA transcript 1 lncRNA PCAT1, SchlAP1 (second chromosome locus associated with prostate-1), and CTBP1-AS indicate cancer cell invasiveness and metastasis in prostate cancer progression. 24531795 LncRNADisease
colorectal cancer qPCR etc. CRC tissue up-regulated N/A Our results showed that PCAT-1 expression in CRC tissues was significantly upregulated compared with the matched normal tissues and the overexpression of PCAT-1 was found in 64 % (62/81) of CRC. In addition, there was a significant association between PCAT-1 expression and distant metastasis. More important, CRC patients with PCAT-1 higher expression have shown significantly poorer overall survival than those with lower PCAT-1 expression. In conclusion, our results suggest that high expression of PCAT-1 is involved in CRC progression and could be a novel biomarker of poor prognosis in patient with colorectal cancer. 23640607 Lnc2Cancer
esophageal squamous cell carcinoma qPCR etc. ESCC tissue up-regulated interaction The expression of PCAT-1 was significantly higher in human ESCC compared with the adjacent noncancerous tissues (70.8 %, p < 0.01), and the high level of PCAT-1 expression was significantly correlated with invasion of the tumor (p = 0.024), advanced clinical stage (p = 0.003), lymph node metastasis (p = 0.032), and poor prognosis. 25731728 Lnc2Cancer
bladder cancer qPCR etc. bladder cancer tissue, cell lines (T24, 5637 etc.) up-regulated expression In this study, we found that PCAT-1 was up-regulated in bladder cancer compared to paired normal urothelium.PCAT-1 plays oncogenic roles. 25934337 Lnc2Cancer
prostate cancer qPCR, Luciferase reporter assay, knockdown etc. cell lines (LNCaP) up-regulated regulation We show that PCAT-1 promotes prostate cell proliferation and that this phenotype is mediated through up-regulation of the cMyc protein (encoded by the MYC gene). Antagonism of cMyc is able to reverse PCAT-1mediated cell proliferation. We show that PCAT-1 regulates cMyc post-transcriptionally through the MYC 3' untranslated region (UTR). Further, we find a protetcive effetc of PCAT-1 on cMyc by interfering with the regulation of MYC by miR-34a. 25425964 Lnc2Cancer
non-small cell lung cancer quantitative real-time PCR (QRT-PCR), suppression non-small cell lung cancer cells up-regulated expression PCAT-1 suppression using PCAT-1 small hairpin RNA (shRNA) with A549 cells inhibited cell proliferation, migration and invasion, while over-expression of PCAT-1 by synthetic plasmid vectors was shown to promote cell proliferation, migration and invasion. Our data suggested that PCAT-1 could play an oncogenic role in NSCLC progression. Silencing PCAT-1 is a potential novel therapeutic approach for lung cancer. 26770456
prostate cancer RNA-seq, qPCR, Western blot etc. prostate cancer tissue, cell lines (VCaP, LNCaP) up-regulated expression PCAT1 is markedly overexpressed in a subset of prostate cancers, particularly metastases, and may contribute to cell proliferation in these tumors. 21804560 LncRNADisease Lnc2Cancer
hepatocelluar carcinoma the reverse transcription-quantitative polymerase chain reaction HCC tissue samples and HepG2 and Bel‑7402 cell lines up-regulated N/A Overexpression of PCAT‑1 induced synthetic plasmid vectors 27035680
 


Interaction
Interaction target Level of interaction Type of interaction Description PMID Source
SUZ12 DNA-TF regulation Chromatin immunoprecipitation (ChIP) assays also demonstrated that SUZ12, a core PRC2 protein, directly binds the PCAT-1 promoter approximately 1kb upstream of the TSS. 21804560 LncRNADisease
EZH2 RNA-RNA co-expression Knockdown of EZH2 by shRNA or pharmacologic inhibition of EZH2 with the inhibitor 3-deazaneplanocin A (DZNep) caused a dramatic upregulation in PCAT-1 expression levels. 21804560 LncRNADisease
PRC2 RNA-Protein binding PCAT-1 is a prostate-specific regulator of cell proliferation and show that it is a target of the Polycomb Repressive Complex 2 (PRC2). Patterns of PCAT-1 and PRC2 expression stratified patient tissues into molecular subtypes distinguished by expression signatures of PCAT-1-repressed target genes. 21804560 LncRNADisease
cMyc RNA-DNA regulation The PCAT-1-cMyc relationship is mediated through the post-transcriptional activity of the MYC 3' untranslated region 25425964
p53 RNA-DNA regulation chromatin immunoprecipitation (ChIP) assays confirmed that PANDAR was a direct transcriptional target of p53 in NSCLC cells. 25731728